Coding

Part:BBa_K3007031:Design

Designed by: Cheng Li   Group: iGEM19_QHFZ-China   (2019-10-16)


Expression device of RinA_p80α under uric acid-responsive regulatory system


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1144
    Illegal EcoRI site found at 2961
    Illegal XbaI site found at 2858
    Illegal SpeI site found at 662
    Illegal PstI site found at 140
    Illegal PstI site found at 2955
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1144
    Illegal EcoRI site found at 2961
    Illegal NheI site found at 830
    Illegal SpeI site found at 662
    Illegal PstI site found at 140
    Illegal PstI site found at 2955
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1144
    Illegal EcoRI site found at 2961
    Illegal BglII site found at 597
    Illegal BamHI site found at 158
    Illegal BamHI site found at 2011
    Illegal BamHI site found at 2875
    Illegal BamHI site found at 2950
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1144
    Illegal EcoRI site found at 2961
    Illegal XbaI site found at 2858
    Illegal SpeI site found at 662
    Illegal PstI site found at 140
    Illegal PstI site found at 2955
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1144
    Illegal EcoRI site found at 2961
    Illegal XbaI site found at 2858
    Illegal SpeI site found at 662
    Illegal PstI site found at 140
    Illegal PstI site found at 2955
    Illegal AgeI site found at 1829
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Figure 1. Schematic cartoon of the DNA construct of BBa_K3007031


Source

E. coli

References